General Cell Lines and Cell Cultures
ACROBiosystems CHO/Human c-MET Stable Cell Line Development Service
Hepatocyte growth factor receptor (HGFR) is also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many ty
STEMCELL Technologies G-CSF and Plerixafor Mobilized Human Peripheral Blood Mononuclear Cells, Frozen, Size: 5 x 10^6 cells
Start your assays with ready-to-use, ethically sourced, mobilized primary mononuclear cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications. Donors are mobilized with G-CSF, plerixafor, or G-CSF and plerixafor prior to collection. Isolated from leukapheresis samples using density gradient separation or red blood cell lysis and cryopreserved in CryoStorCS10 medium, cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. ACDA is added as an anticoagulant. Contact techsupport@stemcell.com for donor specifications (BMI category, smoking status, ethnicity, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Certain products are only available in select territories.
Eurofins Discoverx PathHunter CHO-K1 AGTRL1 β-Arrestin-1 Cell Line, 2 vials. (93-1050C2)
The PathHunter AGTRL1 (ARR1) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTRL1 and EA-tagged β-Arrestin-1. Activation of AGTRL1 stimulates the recruitment of β-Arrestin-1 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Lonza Walkersville Rat Brain Cx-Hi-Cp Mixed Astro
Ready-to-use Clonetics Rat Brain Cortex Astrocytes are obtained from rat brain passaged once and prepared as cell suspensions for shipment on dry ice Glia are a mixture of rat cortical hippocampal and striatal astrocytes Each vial contains approximately 1 million cells Astrocytes can be easily thawed and cultured Astrocytes can be harvested and re-plated or stored frozen for later use The cells are shipped overnight Recommended medium AGM BulletKit (CC-3186)
Lonza Walkersville Adipogenic Induction Medium
Adipogenic Induction Medium is a component of the hMSC Adipogenic Differentiation BulletKit and is availalbe separately Stem cells possess the unique ability to differentiate into multiple lineages of cells including adipocytes Lonza s hMSC Adipogenic Differentiation BulletKit Medium is a serum containing media system designed to induce adipogenic differentiation of human bone marrow derived mesenchymal stem cells into mature functionally active adipocytes in as few as 19 days Differentiation can then be validated by a variety of methods including the use of Lonza s AdipoRed Adipogenic Assay Reagent which provides a quantitative value related to the amount of adipogenic differentiation
Lonza Walkersville D-Hu Subcut Preadipo Diabet I
Poietics Diseased Human Preadipocytes (D-HprAD) are isolated from subcutaneous or visceral fat from diabetic Type I and diabetic Type II donors Preadipocytes from normal (non-diabetic) donors are available as a separate product Pertinent additional donor information including Body Mass Index (BMI) insulin dependence and age of diagnosis may be available by contacting Scientific Support Cryopreserved Poietics Diseased Human Preadipocytes (D-HprAD) are guaranteed to contain 1 million cells per ampoule and test positive for adipogenic differentiation All cells test negative for mycoplasma bacteria yeast and fungi HIV-1 hepatitis B and hepatitis C are not detected for all donors and/or cell lots A Certificate of Analysis is provided for each cell lot purchased
ATCC Influenza B virus; B/Hong Kong/5/72
Influenza B virus strain B/Hong Kong/5/72 is propagated in SPF chicken embryonated eggs. This whole-genome sequenced strain was deposited by the Centers for Disease Control and Prevention and has applications in respiratory and infectious disease research.
ATCC Salmonella enterica; subsp. enterica Typhimurium
Salmonella enterica subsp. enterica serovar Typhimurium is a whole-genome sequenced strain that was isolated in 2017 from Delaware. It has applications in enteric, infectious, and zoonotic disease research.
ATCC Klebsiella pneumoniae; 1000527, 7561
Klebsiella pneumoniae strain 10000527, 7561 was isolated from human urine. This strain was confirmed by PCR to be negative for blaKPC and positive for blaNDM. It has applications for multidrug testing and respiratory research.
Eurofins Discoverx ChemiScreen PK2 Prokineticin Receptor Stable Cell Line, 2 vials. (HTS182C)
The ChemiScreen PK2 Prokineticin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PROKR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of PROKR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PathHunter CHO-K1 GPR120L (Long Isoform) β-Arrestin Cell Line, 2 vials. (93-0366C2)
The PathHunter GPR120L cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of GPR120L GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR120L and EA-tagged β-Arrestin-2. Activation of GPR120L stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx DNA 12K Reagent Kit
DNA Reagent for the DNA 12K LabChip kit. Contains enough reagent for 2000 wells. The kit for use on the LabChip GX and LabChip GX Touch systems provides a fast and easy way to analyze a DNA samples ranging from 100 to 12,000 base pairs, which is an extended size range relative to the companion assays in the PerkinElmer portfolio. Performing DNA sample analysis with the DNA 12K assay saves time and money by automating steps for analyzing size and purity, and replaces agarose slab gels, and imaging. The LabChip GX and GX Touch Electrophoresis Systems uses a single sipper microfluidic chip to aspirate dsDNA samples directly from 96- or 384-well plates. Additional Specifications Sizing Range - 100 bp – 12000 bp Sizing Accuracy - ± 10% Sizing Resolution - ± 10% from 150 – 1000 bp ± 15% from 1000 – 2000 bp ± 20% from 2000 – 8000 bp ± 25% from 100 – 150 bp, 8000 – 12000 bp Linear Concentration Range – 0.25 ng/µL – 50 ng / µL Per fragment Sensitivity – 0.25 ng / µL Chip Sample Lifetime –2000
Cell Applications Inc Proliferating Cardiac Fibroblasts (HCF), Adult
Proliferating Cardiac Fibroblasts (HCF), Adult